Arid1a Knockout cell line(ID8)

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location： Home > Products > KO Cell Lines > Arid1a Knockout cell line(ID8)

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Arid1a Knockout cell line(ID8) Order

Arid1a Knockout cell line(ID8)
Catalog#
YKO-M145
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Arid1a Knockout cell line(ID8)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Arid1a Knockout cell line (ID8 )
Morphology
Epithelial-like, adherent
Passage ratio
1：3-1：4
Species
Mouse
Gene Name
Arid1a
Gene ID
93760
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 3 (based on transcript Arid1a-205)
Validation: PCR+Sanger Sequencing
Arid1a Knockout cell line(ID8) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:ID8		 STR Info (Cell bank)
Cell Line:ID8
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 17.017.0
4-2 19.319.3
6-7 15.015.0
19-2 13.013.0
1-2 20.020.0
7-1 26.226.2
8-1 16.018.0
1-1 16.016.0
3-2 14.014.0
2-1 16.016.0
15-3 19.222.322.3
6-4 18.018.0
13-1 17.117.1
11-2 16.016.0
17-2 15.015.0
12-1 17.017.0
5-5 17.017.0
X-1 27.027.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Arid1a gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the ID8 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Arid1a gene knockout Arid1a Knockout cell line (ID8 ) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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